Abstract
Objective: To investigate the effect of IL-10 on the inducibility of human β-defensin-2 (hBD-2) in human peripheral blood cells. Methods: Peripheral blood samples were collected from 22 healthy individuals and co-cultured with 0ng/ml lipopolysaccharide (LPS), 100ng/ml LPS, 10ng/ml IL 10, or 100ng/ml LPS plus 10ng/ml IL-10 at 37℃ for 6h. Total RNA was extracted from peripheral blood cells and the mRNA level of hBD-2 was detected by relative quantitative real-time PCR. Results: No detectable level of hBD-2 mRNA was found in normalperipheral blood cells with stimulation of 0ng/ml LPS or 10ng/ml IL-b. The mRNA level of hBD-2 was increased to 166.9±35.14 after 100ng/ml LPS challenge, while the mRNA level of hBD-2 was decreased to 30.40±9.18 after the co-stimulation of 100ng/ml LPS plus 10ng/ml IL-10; which was significantly lower than that with LPS alone (P＜0.05). Conclusion: The expression of hBD-2 gene can be induced by LPS. IL-10 inhibits the inducible expression of hBD-2.
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