Changes of mn23-H1 gene expression in human peripheral blood cell after X-ray irradiation

Abstract

Objective To investigate the expression change of nm23-H1 gene in human peripheral blood cells exposed to ionizing irradiation.and explore the feasibility of nm23-H1 gene expression changes as a biodosimeter.Methods Human peripheral whole blood samples were collected from four healthy individuals.and exposed to X-rays with the doses between O and 5 Gy.The total mRNAs were isolated from the white-blood cells,and the changes of nm23-H1 gene mRNA expression postirradiation were detected by real-time RT-PCR at 0,6,12 and 24 h,respectively.Results The levels of nm23-H1 gene expression in human peripheral blood cells increased gradually with the doses from l to 3 Gy at 6 h(F=15.064,P 0.05).The time-effect curve showed that the expression of nm23-H1 gene decreased with the doses of O,4 and 5 Gy after irradiation (F=8.636,4.112,5.411,P<0.05).Meanwhile,the expression of nm23-H1 gene showed the difference only at 12 h with 1 Gy irradiation (t=-2.527,P<0.05)and was the lowest at 24 h with 2 and 3 Gy irradiation (F=12.517,6.622,P<0.05).Conclusions The expression change of nm23-H1 gene could be induced by X-ray in human peripheral blood cells,and might be a potential biodosimeter candidate applicable in early-response radiation accident victims. Key words: X-rays; nm23-H1; Gene expression changes; Biodosimeter