102 Nutrition Affects Tendon Healing In A Rat Model

Abstract

Introduction We have previously shown that human and rat tendon cells produce insulin and secrete it upon glucose stimulation. Moreover, the level of tendon insulin production is affected by the amount of glucose taken up by nutrition [Lehner, 2012]. We now hypothesise that nutritional glucose affects tendon healing in a rat model. Methods In 60 female Lewis rats full thickness defects were created in one Achilles tendon and left unsutured. The rats were randomly assigned to three groups, one was fed a high glucose diet, one a diet with low glucose/high fat and one a control diet, for 2 weeks each. Before surgery, one and two weeks after, gait analysis was performed using a Noldus TM catwalk system. After two weeks the animals were sacrificed and tendon size was measured and tendons were biomechanically tested an evaluated by various histological methods. Results Gait Analysis revealed a significant difference between the three groups one week after surgery. The intermediate toe spread (the range between second and fourth toe, a measure for the load on the limb) of the high glucose group is significantly increased one week p.o. (0,49 cm ± 0,07; n = 20; p Measurement of length and thickness of the newly formed tissue revealed a significant (p Biomechanical testing revealed no significant difference between the groups in maximum tensile load, however, the new fibrous tissue from the glucose group is significantly (p Discussion Newly formed tendon tissue quality is affected by nutritional glucose. This finding is relevant for understanding diabetes related tendinopathy. Nutritional parameters may account for the interindividual variation of tendon quality and regeneration. The underlying molecular mechanisms will be examined. Reference Lehner et al . Horm Metab Res. 2012;44:506–510