1005 THE FUNCTIONAL CONSEQUENCES OF ALLOGENICITY OF ADIPOSE DERIVED STEM CELLS FOR INTRACAVERNOSAL INJECTION AFTER CAVERNOUS NERVE INJURY IN RATS

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research II1 Apr 20121005 THE FUNCTIONAL CONSEQUENCES OF ALLOGENICITY OF ADIPOSE DERIVED STEM CELLS FOR INTRACAVERNOSAL INJECTION AFTER CAVERNOUS NERVE INJURY IN RATS Naside Mangir, Cem Akbal, Ferruh Simsek, Tufan Tarcan, and Levent Turkeri Naside MangirNaside Mangir Istanbul, Turkey More articles by this author , Cem AkbalCem Akbal Istanbul, Turkey More articles by this author , Ferruh SimsekFerruh Simsek Istanbul, Turkey More articles by this author , Tufan TarcanTufan Tarcan Istanbul, Turkey More articles by this author , and Levent TurkeriLevent Turkeri Istanbul, Turkey More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.1107AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Intracavernous injection of adipose derived stem cell(ADSC)s can ameliorate erectile functions after cavernous nerve injury in rats. The use of pre- prepared allogenic stem cells are desired but no comparative studies exist. The aim of this study is to evaluate functional consequences of either allogenic or autologous use of adipose derived stem cells. METHODS All rats underwent bilateral paratesticular fat tissue excision at the beginning of the study. ADSCs are isolated from the excised adipose tissue in Marmara University School of Medicine Urologic Research Laboratory. After 15 days of fat excision bilateral cavernous nerve injury is performed which is followed by intracavernosal injection of stem cells or stem cell lysates accordingly to each group. One month after injection Intracavernosal Pressure/ Mean Arterial Pressure(ICP/ MAP) values are calculated after intracavernosal pressure measurement. All rats are sacrificed at the end and penile tissues are taken for immunohistochemical and molecular investigation. RESULTS A total of 36 Sprague Dawley rats were used. ADSCs isolated from the excised paratesticular fat tissue were characterized with flowcytometry and these cells were demonstrated to be CD90 and CD 44 positive; CD11b, CD 34 and CD45 negative. These stem cells were also differentiated into 2 different tissues to prove their multilineage differentiation capacity. One month after injection ICP/ MAB values in the sham group (Group 1) were significantly higher compared to PBS injection (Group 2) group, 73,1 (±10,3) ve 47,1 (±14,2) (Mann Whitney U test p: 0,009), respectively. Both autologous stem cell injection group (Group 5) and allogenic stem cell injection group (Group 3) showed statistically insignificant but still better results when compared to PBS injection (Group 2), 62,1 (±12,3), 61,0 (±10,6) and 47,1 (±14,2) respectively. Autologous or allogenic stem cell lysate injections(Group 4 and 6) were not better than PBS injection. The nNOS staining in corpus cavernosum of rats treated with autologous or allogenic stem cells were stronger. Also nNOS expression was quantitatively better in stem cell injection groups. CONCLUSIONS ADSC injection can be an effective treatment to regain erectile functions after cavernous nerve injury. The use of either autologous or allogenic stem cells does not change the functional results. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e409 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Naside Mangir Istanbul, Turkey More articles by this author Cem Akbal Istanbul, Turkey More articles by this author Ferruh Simsek Istanbul, Turkey More articles by this author Tufan Tarcan Istanbul, Turkey More articles by this author Levent Turkeri Istanbul, Turkey More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...