MP52-01 NANOTECHNOLOGY IMPROVED ADIPOSE DERIVED STEM CELL THERAPY IN ERECTILE DYSFUNCTION IN POST RADICAL PROSTATECTOMY ANIMAL MODEL

Abstract

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Basic Research I1 Apr 2015MP52-01 NANOTECHNOLOGY IMPROVED ADIPOSE DERIVED STEM CELL THERAPY IN ERECTILE DYSFUNCTION IN POST RADICAL PROSTATECTOMY ANIMAL MODEL Haocheng Lin, Nadeem Dhanani, Hubert Tseng, Glauco Souza, and Run Wang Haocheng LinHaocheng Lin More articles by this author , Nadeem DhananiNadeem Dhanani More articles by this author , Hubert TsengHubert Tseng More articles by this author , Glauco SouzaGlauco Souza More articles by this author , and Run WangRun Wang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.1718AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Recently, intracavernous injection (ICI) of stem cells has shown some promise for erectile dysfunction (ED). However, most stem cells were washed out immediately due to the communication between corpus cavernosum (CC) and the blood circulation. We previously reported a novel nanotechnology to keep adipose derived stem cells (ADSCs) in CC in animal model. The present study was designed to investigate the effect of this therapy on erectile dysfunction by cavernous nerve injury. METHODS Adipose-derived stem cells (ADSCs) were isolated from inguinal fat tissue of adult male Sprague–Dawley (SD) rats and cultured in DMED/F12 medium. NanoShuttle were added to ADSCs. ED animal models were created by bilateral cavernous nerve crush (BCNC) injury and randomly assigned into four groups (n=6). Group A: BCNC. Group B: ADSCs ICI. Group C: ADSCs with NanoShuttle ICI. Group D: ADSCs with NanoShuttle ICI + magnetic probes. Intracavernous pressure / Mean arterial pressure (ICP/MAP) was recorded under cavernous nerve stimulation at day 28 after ICI. Then, rat penes were harvested and processed for immunohistology and western blot of platelet endothelial cell adhesion molecule (PECAM-1) and alpha smooth muscle Actin (α-SMA). RESULTS Group B, C and D improved erectile function in rats after BCNC injury. Group D had a significant better effect than Group B and Group C (P<0.05). Group B, C and D significantly increased the expressions of PECAM-1 and α-SMA in CC compared with Group A (P<0.05); while Group D had a significant higher expression than Group B and Group C (P<0.05). CONCLUSIONS NanoShuttle plus magnetic probe improved ADSCs therapy for ED in nerve injury animal model. © 2015 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 193Issue 4SApril 2015Page: e628 Advertisement Copyright & Permissions© 2015 by American Urological Association Education and Research, Inc.MetricsAuthor Information Haocheng Lin More articles by this author Nadeem Dhanani More articles by this author Hubert Tseng More articles by this author Glauco Souza More articles by this author Run Wang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...