Abstract
This chapter describes the techniques that permit measurement of fluorescence emission on the picosecond time scale. Some biological processes are extremely fast. One such process is the photochemical event by which visual pigments absorb a photon of light and convert this energy to chemical energy. Rhodopsin, the name given to visual pigment rod proteins, is photochemically converted to the high-energy species, called bathorhodopsin, within a picosecond. Most molecular decay routes take place in the order of tens of picoseconds or longer. Chromophore photoisomerization plays a central role in the primary photophysics of rhodopsin. Quite different fluorescence kinetics and quantum yields are obtained for analogs where the C-l 1–C-12 double bond is rotationally hindered.
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