1 - Peptide synthesis: Methods, trends, and challenges

Abstract

This chapter provides an overview of peptide synthesis giving emphasis on solid-phase peptide synthesis (SPPS). Currently, most of the peptides used for research or therapeutics are synthesized by SPPS methods. Successful peptide synthesis overcoming the usual handles and limitations is extremely dependent on the convenient selection of resins, linkers, amino acid derivatives, and coupling reagents as well as side chain (de)protection and cleavage conditions. Old and newer solid supports, linkers, protecting groups, coupling reagents, deprotection, and/or cleavage solutions widely used in Fmoc/tBu methodology are presented and discussed. Newer greener ways of synthesis are also presented. The chapter concludes with future challenges, perspectives and opportunities in the field of solid-phase peptide chemistry.