Abstract
Publisher Summary Even the most superficial perusal of the literature for the purpose of reviewing high-performance liquid chromatography (HPLC) separations of peptides quickly reveals that shortage of relevant material is certainly not a problem. This is due primarily to the tremendous development of high-performance chromatographic techniques, in the past few years, in terms of scale, instrumentation, and column packings. In addition, there is an almost bewildering variety of mobile phases, employed by various researchers for specific applications in all major modes of HPLC employed for peptide separations. This chapter discusses the laboratory-based researchers, both beginners and more experienced chromatographers, who wish to learn about peptide applications in HPLC. Thus, standard analytical applications in HPLC of peptides are stressed, as opposed to micro- or preparative-scale chromatography. Only nonspecialized columns, mobile phases, and instrumentation readily available and easily employed by the researcher are described in detail in the chapter. In addition, through the use of peptide standards specifically designed for HPLC, this chapter describes the standard operating conditions that should first be attempted in the separation of a peptide mixture.
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