Abstract
Collagenase clostridium histolyticum (CCH, Xiaflex® (Endo Pharmaceuticals, Inc., Malvern, PA, USA) is an effective and safe minimally invasive intralesional therapy option for Peyronies disease (PD). A previous clinical study published by our group showed a negligible effect of CCH on penile vascular parameters, or the International Index of Erectile Function score in PD patients who completed four rounds of CCH when compared to baseline. Although PD is strongly associated with erectile dysfunction, there are no studies related to the understanding of such effects of CCH on human corpus cavernousm (HCC) smooth muscle function. To explore the effects of different concentrations of CCH on nitric oxide (NO)-dependent and -independent nitrergic relaxations of HCC in organ bath studies in order to understand if CCH can improve HCC relaxation and thus ED in PD patients. HCC samples were obtained from men undergoing penile prosthesis implantation (n = 10). After phenylephrine (Phe) contraction, electrical field stimulation (EFS), and acetylcholine (ACh)-induced relaxation at [0.23 and 0.9 mg] CCH incubations of HCC strips were performed using organ bath preparations. HCC measurements of endothelial NO synthase (eNOS), neuronal (nNOS), and vascular endothelial growth factor were evaluated through immunostaining and Western blotting.
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