Abstract
It has been postulated that elevated levels of matrix metalloproteinases (MMPs) and reactive oxygen species detected in fluids and biopsies of chronic wounds contribute to the failure of wound to heal by degrading essential growth factors, receptors and extracellular matrix proteins. This stimulated the development of a formulation containing metal ions (polyhydrated ionogen or PHI) and citric acid that reduced reactive oxygen species in cultures of polymorphonuclear leukocytes (J Wound Care 12:10, 2003). The effect of PHI on synthesis of MMPs was studied in cultures of tumor cells and human dermal fibroblasts. Dose response curves of PHI ranging from 0.02% to 10%(W/V) established no toxicity in cultures of human dermal fibroblasts after 24 hours of incubation with PHI at concentrations below 1.25%, as measured by MTT assay. Levels of MMP-2 activity measured by antibody capture assay were reduced approximately 80% in culture medium of Mor-86 glioma tumor cells after 24 hours of exposure to 1% PHI. These results suggest that PHI reduced the synthesis of MMP-2 protein, presumably by reducing transcription and/or translation of MMP-2 gene. Clinical case reports of chronic wounds treated by PHI dressing (DerMax) suggest that PHI treatment stimulated healing and reduced MMP-2 immunostaining in biopsies of the chronic wounds. Acknowledgment: This research was supported by Greystone Medical Group.
Published Version
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