074 Action of an ORC/Collagen Matrix Containing Silver on the Prolonged Inflammatory Process in Chronic Wounds

Abstract

The inflammatory phase of wound healing performs vital acts of host defense, removing invading microorganisms and or devitalized tissue. Characterized by an infiltration of neutrophils and monocytes, within hours of injury inflammation begins to effectively clear the wound in a strictly regulated manner in preparation for the following stages of repair and regeneration. Chronic wounds are varied in their etiology, resulting from many underlying pathologies. However, collectively they are often described as being in a persistent state of inflammation. Production of proinflammatory cytokines, proteinases and reactive oxygen species (ROS) in this unregulated manner can cause significant damage. By stopping the action of proinflammatory cytokines, further recruitment of neutrophils may be diminished, helping to stop protease production at its source. Chronic wound dressings that are able to help interrupt the self- perpetuating process may help to create the optimal wound healing environment. In this study we investigated the impact of silver and then an ORC/collagen matrix containing silver-ORC on the production of proinflammatory cytokines by lipopolysaccarhide (LPS) stimulated monocyte macrophages (THP-1 cells), an in vitro model of inflammation. The ability of the ORC/collagen matrix containing silver-ORC matrix to quench free radicals was also investigated. Results show that the ORC/collagen matrix containing silver-ORC is capable of reducing the levels of proinflammatory cytokines. This dressing also has antioxidant activity, quenching ROS. These influences on the biochemistry of a chronic wound may have an antiinflammatory action, limiting the destruction of new tissue and promoting wound closure.