高度糖化終產物受器基因多型性與 sRAGE，慢性牙周炎分別在非糖尿病與糖尿病台灣人之相關性

Abstract

Background and Objective: The present study investigated the association between the RAGE G82S、-374 T/A、-429 T/C polymorphisms, the plasma levels of sRAGE and chronic periodontitis (CP) in subjects with and without diabetes mellitus (DM). Material and Methods: A total of 208 DM patients and 260 non-DM participants were recruited for this study. Genotyping of the RAGE G82S、-374 T/A、-429 T/C polymorphisms were accomplished using PCR-RFLP, and associations were analyzed with the chi-square test and logistic regression analysis. Results: In the non-DM group, the chi-square test showed that the frequency distributions of the G82S polymorphisms were significantly different between CP and non-CP subjects (χ2 = 4.6, p = 0.04). A multivariate logistic regression model showed that the G82S+S82S genotypes were associated with a significantly increased risk of CP development compared to the G82G genotype (adjusted odds ratio (OR) = 2.03, 95% confidence interval (CI): 1.07- 4.00). In the DM group, there was no association between the G82S polymorphism and CP development when a multivariate logistic regression was performed. In total, non-DM and DM group, there were no association between the RAGE -374 T/A、-429 T/C polymorphisms and CP development when a multivariate logistic regression was performed. There was no difference in plasma sRAGE levels between CP and non-CP subjects in total, DM and non-DM groups. Plasma levels of sRAGE were significantly higher in subjects with the G82G genotype compared to those with the G82S +S82S genotypes in both the non-DM (857.0 ± 333.4 vs. 728.5 ± 312.5 pg/ml, p< 0.0001) and DM groups (901.9 ± 500.8 vs. 616.5 ± 301.9 pg/ml, p < 0.0001). There were no association between -429 T/C、 -374 T/A polymorphisms and plasma sRAGE levels. Plasma levels of sRAGE were significantly lower in DM group compared to non-DM group in subjects with the G82S+S82S genotypes (616.5 ± 301.9 vs. 728.5 ± 312.5 pg/ml，p<0.03). Conclusions: The present study revealed that the RAGE G82S polymorphism was associated with chronic periodontitis in the non-DM group but not in the DM group. Our results also showed that the plasma levels of sRAGE were significantly higher in subjects with the RAGE G82G genotype, and this correlation was not affected by the presence of CP in the DM and non DM groups.