Abstract
“Crude” extracts obtained by ultrasonic disintegration of microorganisms are promising bioelectrocatalysts. Extracts from Saccharomyces cerevisiae are of particular interest because they may contain a significant number of enzyme systems that oxidize simple carbohydrates such as glucose. A technology for producing “crude” protein extracts of Saccharomyces cerevisiae has been developed. The percentage of glucose added to the nu-trient medium, as well as the composition of the buffer solution at the resuspending stage, the power and time of ultrasonic disintegration of biomass, the rate and time of centrifugation during separation of the sediment of destroyed cells from the filler fluid have a significant effect on the enzymatic activity of the resulting “crude” extract.
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