S ubtercola

Abstract

Sub.ter'co.la. L. prep. subter below, underneath; L. masc. suff. ‐ cola inhabitant; N.L. masc. n. subtercola the one who lives underneath. Actinobacteria / Actinobacteria / Micrococcales / Microbacteriaceae / Subtercola The genus Subtercola is phylogenetically placed within the radiation of the actinomycete genera with group‐B‐peptidoglycan that comprises the family Microbacteriaceae . The genus is closely related to the genus Agreia . Cells form short irregular rods, are Gram‐stain‐positive, nonencapsulated, nonmotile, and nonsporeforming. Aerobic. The colonies are circular, convex, smooth, and pale to bright yellow, depending on the medium. Growth occurs best at 15–17°C, but is also observed in the temperature range 2–28°C. The cell‐wall diamino acid is diamino butyric acid (DAB). The main cell‐wall amino acids are alanine, glycine, threo ‐3‐hydroxy‐glutamic acid, and DAB. The peptidoglycan type is B2γ. The polar lipids are phosphatidylglycerol, diphosphatidylglycerol, one unknown phospholipid, and two glycolipids. The main cellular fatty acids are 12‐methyltetradecanoic acid (C 15:0 anteiso), 14‐methylpentadecanoic acid (C 16:0 iso), and 14‐methylhexadecanoic acid (C 17:0 anteiso). The whole‐cell methanolysates of Subtercola species contain 1,1‐dimethoxy‐anteiso‐pentadecane (C 15:0 anteiso DMA) and 1,1‐dimethoxy‐iso‐hexadecane (C 16:0 iso DMA) as major components in amounts comparable to those of fatty acids. No mycolic acids are present. The major isoprenoic quinones are MK‐9 and MK‐10. The genomic DNA G+C content is 64–68 mol%. DNA G + C content ( mol% ): 64–68. Type species : Subtercola boreus Männistö, Schumann, Rainey, Kämpfer, Tsitko, Tiirola and Salkinoja‐Salonen 2000, 1737 VP .