Abstract
The destruction of aromatic hydrocarbons by the Rhodococcus wratislaviensis KT112-7 strain isolated from technogenic mineral waste products of the BKRU1 Uralkalii factory has been investigated (city of Berezniki, Perm krai). The R. wratislaviensis KT112-7 was shown to utilize increased concentrations of ophthalic (o-PA) (8 g/L) and benzoic (BA) (3.4 g/L) acids. The strain grows with o-FA, BA, and biphenyl at a NaCl content of up to 50, 90, and 75 g/L in the culture medium, respectively. Based on an analysis of the metabolic profile and nucleotide sequences of the bphA1, benA, and phtB genes, the KT112-7 strain was established to decompose o-PA via the formation of 3,4-dihydroxyphthalic and 3,4-dihydroxybenzoic acids. The decomposition of biphenyl is carried out via the formation of BA and then at low concentrations of NaCl (up to 50 g/L) via the formation of 4-hydroxybenzoic acid followed by its oxidation; at high concentrations of NaCl (over 60 g/L), via the direct oxidation of benzoic acid with the production of catechol. These data indicate that the Rhodococcus wratislaviensis KT112-7 destructor strain is a promising strain for the development of new biotechnologies directed at the utilization (transformation) of aromatic compounds, including under the conditions of increased mineralization.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.