Abstract
The 107 maize samples, collected from stores, retailers and supermarkets, were used for serial tests of genetic modified organism (GMO). Total 321 test samples were picked from these 107 maize samples. First test was performed by Multiplex PCR, using primers designed from 35S Promoter, NOS Terminator and zein, to identify GMO maize. Then, GMO maize was further identified for their types by using the method of recognizing eight different types of GMO maize, published in 2005 by Onishi. In the detection of different types of GMO maize, 71.4% test samples was detected to be NK603. The test samples, detected to be GMO maize, will be quantity. There are disadvantages in the past way in using QCPCR, such as that the size of PCR products between competitor and target should be large enough to be separated on agarose gel. The image took to analysis will also be affected by factors like staining time of EtBr and the system used to capture the image. Therefore, we try to overcome those problems by taking HPLC into QCPCR. A reference plasmid, applied by Yang to real-time PCR for nine specific types of maize, was modified to form a standard plasmid for the test of QC-PCR. The modified plasmid, inserted with 6 bp restriction site, specific for the use in quantitation of NK603, could be put into comparing real-time PCR and QC-PCR equally. The two peaks could be separated in 40 seconds by HPLC system in this condition: 5 mM NaCl gradient per min and 0.75 mL per min flow rate. HPLC system will further use in rapid, repetitive and accurate quantitation of GMO.
Published Version
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