福壽螺 (Pomacea canaliculata) 的生理生化值與農藥之相關性

Abstract

The Apple snail (Pomacea canaliculata) is a common freshwater snail in the paddy fields. Objective of this research is to find out if Apple snails would be a suitable model organism in monitoring the value of the pesticide in the paddy fields. Samples of this research were collected from organic and non-organic paddies, using chemicals during farming time, at a week before rice transplanting and two weeks before harvesting in the western part of Taiwan. Five sample sites were selected, Hsinchu, Yunlin, Tainan and Pingtung T are non-organic paddies except Pingtung O. We monitored the monooxygenase (MO), glutathione-S-transferase (GST), aspartate aminotranssferare (GOT/AST), alanine aminotransferase (GPT/ALT) and vitellogenin (VTG) in apple snails, and use apple snails which are not polluted by chemicals as the control. All of the MO and GST values in the collected apple snails are higher than those of the control except for the value of Crea. Most values measured in Pingtung T, third crops of rice harvesting area, are higher than the other sites. Besides, because of the diversity pesticides used in different timing during rice growth, the MO, GST, AST and ALT testing also have different reactions. The VTG study in apple snails shows that except for the control and the samples collected in organic paddies, the VTG is induced in male individuals of the apple snails from other study sites. All of the biochemical parameters we tested are active in the apple snails in this study, particularly the MO, GST and VTG tests. According to this study, the biochemical parameter tests of MO, GST and VTG in apple snails are fine tests in detecting the usage of pesticides. Hence, the apple snail can be regarded as a valid model organism to monitor agricultural activities by testing its biochemical parameters.