Abstract
PLA₂ is the second highest content of bee venom components next to the melittin. However, unlike melittin has various pharmacological effects, PLA₂ has been known as an allergen and thus most researches on PLA₂ were focused on removing it or inhibiting its activity. Recently, PLA₂ is effective in the treatment of liver damage and cancer cell suppression. PLA₂ is in the spotlight as an active ingredient of pharmaceuticals. In this study, we developed a method for effective isolation of PLA₂ from bee venom. Sep-pak cartridge column was used to separate pure PLA₂ from the TFA / MeCN mobile phase after 6 steps including column stabilization and bee venom injection. The purity was 99.7±0.03% and the yield was 37.5±3.6%, proving that PLA₂ of high purity can be effectively separated by this method. Purified PLA₂ activity, as well as standard PLA₂ activity had the growth inhibitions against hepatocellular carcinoma cells. This study will be useful for the research on physiological activity of PLA₂ and the development of various pharmaceuticals containing PLA₂.
Published Version
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