Эффект озона на кислородтранспортную функцию и прооксидантно-антиоксидантный баланс крови в условиях воздействия на NO-генерирующую систему в опытах in vitro

Abstract

Ozone has a wide range of physiological effects, in particular, it improves the rheological properties of blood and affects oxygen-dependent processes. The purpose of this study is to investigate the effect of ozone on blood oxygen transport function and prooxidant-antioxidant balance in under conditions of changing nitrogen monoxide formation in vitro experiments. The study used an ozone concentration of 6 mg/l and drugs that change affecting the synthesis of nitrogen monoxide in erythrocytes (nitroglycerin, L-arginine and L-NAME). Revealed that the introduction of the nitroglycerin leads to increased effect of ozone on the oxygen-transport function (manifested by increase in pO2, SO2, p50real) and increase concentration of gaseous transmitters NO, H2S. When adding L-arginine, L-NAME, and a combination of these drugs to experimental blood samples under the influence of ozone, changes in the studied parameters were not detected. The impact on the NO-dependent mechanisms of the intra-erythrocyte system did not significantly affect the processes of lipid peroxidation (malondialdehyde, diene conjugates) and antioxidant protection (catalase, retinol, α-tocopherol).