Abstract
Intracallus and intercallus morphological variability of doubled haploids of rice Oryza sativa L. obtained in androgenesis in vitro on callus lines with multiple regeneration was studied. Regenerants from hybrids of the second generation F2 were used: Don 4237 × (Szarvasi 70 × Heilunjiang)—D × S × H, plant no. 8; Romanika × (Darius 122 × Krasnodar 9167)—R × D × 67, plant no. 15; Kitaets × (VNIIR 3223 × Kenzo)—K × 23 × K, plant nos. 26 and 28. Seed offspring of doubled rice haploids of the first generation R1 were sown on a plot in 1.54 m2 vessels filled with field soil. In total, 1383 plants of 144 lines of doubled haploids of eight callus lines were analyzed. Analysis of variance found statistically significant differences between doubled haploids of two callus aggregates of the same anther and different anthers of the same hybrid. A correlation dependence (p < 0.05) of a number of biometric parameters on plant height was found: panicle length (r = 0.72), grain mass of the main panicle (r = 0.80), plant grain mass (r = 0.59), mass of 1000 grains (r = 0.74). Thus, tall plants turned out to be more productive than low and medium-sized ones. After an initial assessment of the lines of doubled haploids by biometric parameters, the seed offspring of the most productive plants of one callus line were combined to increase the sample volume and its rapid reproduction. This step will make it possible to move more quickly from considering the productivity of individual plants to the field assessment of productivity.
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