Abstract
This paper aims to study the different T-helper subpopulations in peripheral blood as well as the expression of TBX21, RORC, GATA3, NFKB1, MAPK8 and STAT3 genes responsible for the regulation of T-helper differentiation in persons chronically exposed in utero. The object of the study was peripheral blood cells taken from people (156 persons) chronically exposed to radiation in utero and in the postnatal period in a wide range of doses on the Techa River. The mean cumulative absorbed dose to red bone marrow in the examined exposed individuals was 49651.2 mGy (dose range: 73.5-1298 mGy), in the comparison group 1 – 18.71.97 mGy (dose range: 0.78-57 mGy), in comparison group 2 (exposed only postnatally) – 57149.1 mGy (dose range: 86.74-1240 mGy). The subpopulation composition of T-helper cells was analyzed by flow cytofluorometry. The relative mRNA content of TBX21, RORC, GATA3, NFKB1, MAPK8 and STAT3 genes was assessed by PCR-BP. A dose-dependent decrease in the total number of T-helper cells, effector memory T-helper cells and central memory T-helper cells at the trend level and an increase in the relative number of native T-helper cells in the peripheral blood of individuals exposed in utero and postnatally were found. An increase in the relative number of type 1 T-helper cells was also revealed in those exposed in utero and postnatally relative to the group of persons exposed only postnatally. These changes did not depend on the accumulated radiation doses. There were no statistically significant changes in the mRNA expression of the studied genes (GATA3, STAT3, TBX21, MAPK8 and RORC). No correlation between the mRNA expression of the studied genes and the relative number of cells in the subpopulations of T-helper types 1, 2 and 17 in the examined individuals was revealed.
Published Version
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