Abstract
Effect of filtrate of culture medium containing mycelia of Neurospora crassa on the perithecial formation has been studied. The filtrate of medium containing mycelia of a single sexual strain of either mating types was less effective compared with that of medium containing mycelia of both mating types in stimulating the perithecial formation. The effect of the mixed culture filtrate surpasses the additive effect of two single culture filtrates of each mating type. This seems to indicate the presence of cooperative unknown other factor. In fact, the single culture filtrate of one mating type stimulates the formation of perithecia on mycelia of the opposite mating type; these perithecia, however, have failed to produce asci and ascospores. Consequently, it may be concluded that the formation of perithecia on the haploid vegetative thallus of one mating type are stimulated by diffusible substance secreted by mycelia of the opposite mating type, quite irrespective of the sexual fusion of nuclei of both mating types.
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