Induction of insensitive peanut somatic embryo to medium containing culture filtrate from various races of Sclerotium rolfsii and plantlet regeneration

Abstract

The experiment aimed to induction of insensitive peanut somatic embryo (SE) to culture filtrate medium from various races of S. rolfsii and its plantlet regeneration. To achieve these objective, the experiments have been done in several steps: somatic embryo development, preparation of culture filtrate (CF) medium from various races of S. rolfsii, induction of insensitive peanut somatic embryo and peanut plantlet regeneration of insensitive somatic embryo. Result of experiment showed that selected SEs on selective medium containing culture filtrate of S. rolfsii one race or two races or three races or four races were specifically insensitive only on selective medium containing culture filtrate one race or two or three or four races of S. rolfsii. All SEs selected in culture filtrate medium of four races S. rolfsii (race 1, 6, 8, and 9) were more insensitive on culture filtrate medium of S. rolfsii races compared with SEs that only selected in culture filtrate medium of one race. Selected insensitive SEs had ability to form normal seedling (50.5-65.5%) and abnormal germinated SEs (23.5 - 30.5%). Normal germinated SEs developed to form plantlets. These plantlets were developed to produced peanut plant R1 generation and peanut seeds. Further evaluation using subsequent generation of peanut (R1 and R2) plants need to be conducted to testing peanut line resistance to stem rot disease (S. rolfsii) in the field.