α-Cyperone Ameliorates Lipopolysaccharide-Induced Inflammation and Apoptosis of Periodontal Ligament Stem Cells via Blocking the Toll-Like Receptor 4/NF-κB Signaling

Abstract

Periodontitis is a long-lasting inflammatory microbial sickness that could cause damage to the periodontal ligament, gums, cementum and alveolar bone directly. Cyperus rotundus is a common traditional Chinese medicine clinically with many pharmacological activities, α-Cyperone is a bioactive ingredient abundant in Cyperus rotundus, few studies have focused on its anti-fungal and anti-oxidative stress activities in mice, during this research, we firstly investigated the impact of α-Cyperone on PDLSCs inflammatory by LPS stimulation. Our findings illustrated that α-Cyperone exerted no influence on PDLSCs viability at concentrations lower than 60 μM, and the release of inflammatory cytokines decreased with an increase in LPS concentration. Meanwhile, we found that α-Cyperone effectively restrained LPS-induced PDLSC cell apoptosis by CCK-8 cell viability assay and TUNEL-positive staining. Furthermore, the TLR4 expression and NF-κB kinase activation were greatly repressed by α-Cyperone treatment in LPS-induced PDLSCs inflammatory model. Subsequently, enhanced TLR4 expression was observed to reverse the suppressed release of inflammatory cytokines and decreased apoptosis effects mediated by α-Cyperone in LPS-cotreated PDLSCs, indicating that the deactivation of TLR4 and downstream NF-κB were implicated in favoring α-Cyperone-triggered protective effects of LPS in PDLSCs.