Abstract
Abstract Context.—β-Catenin is an adhesion molecule that also plays a role in the Wnt signaling pathway. Objective.—To analyze β-catenin mutation and accumulation in a series of liposarcomas and malignant fibrous histiocytomas. Design.—β-Catenin mutation in exon 3 was studied using polymerase chain reaction–single-strand conformation polymorphism (PCR-SSCP) and direct sequencing analysis in 30 formalin-fixed, paraffin-embedded liposarcomas. The tumors included 12 dedifferentiated liposarcomas, characterized by both high-grade anaplastic components and well-differentiated liposarcoma components, plus 18 well-differentiated liposarcomas (10 lipoma-like and 8 sclerosing-type cases). The 2 components of dedifferentiated liposarcomas were analyzed independently. β-Catenin accumulation in the nuclei or cytoplasm and Ki-67 expression (cell-proliferation marker, MIB-1 labeling index) were examined immunohistochemically. Nine storiform-pleomorphic–type malignant fibrous histiocytomas were also studied. Results.—De...
Published Version
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