Abstract
This study was conducted to investigate the supplementary effects of vitamin C and E to moist pellet on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Four groups of diet made : moist pellet (MP) diet group was used as the control group where the ratio of raw feed and commercial compound meal is 5:5 <TEX>$(CP\;30\%,\;CL\;17\%),$</TEX> vitamin C added group (VC), vitamin I added group (VE) and vitamin C and I added group (VCE). The supplementary amount of vitamin C and E corresponded to 1000 mg/kg (dry wt.) and 220mg/kg (dry wt.), respectively. Vitamin C was destroyed <TEX>$50\%$</TEX> and vitamin E was destroyed <TEX>$20\%$</TEX> for manufacturing process. After 8 weeks feeding trial, weight gain was <TEX>$121.9\%$</TEX> in MP group, while it ranged from 180.5 to <TEX>$184.9\%$</TEX> in the VC, VE and VCE group. Feed efficiency was <TEX>$71.\6%$</TEX> in MP group, whereas it ranged from 78.7 to <TEX>$80.6\%$</TEX> in the VC, VE and VCE group, weight gain and feed efficiency of fish fed vitamin supplemented diets were significantly higher than control group and no significant difference among the vitamin added groups was observed (p>0.05). There were no significant differences in moisture, crude protein, crude lipid, crude ash, condition factor (CF), hepatosomatic index (HSI), and visceraweight index (VWI) of body composition. Hemoglobin (Hb) was <TEX>$3.79\%$</TEX> in MP group, whereas <TEX>$4.99\%$</TEX> in VC group, <TEX>$4.55\%$</TEX> in VE group and <TEX>$5.07\%$</TEX> in VCE group; Hb was significantly higher in the vitamin added groups than the control group (p<0.05). Vitamin C retention in liver was 18.1, 20.7, 46.6, 54.0 mg/kg in MP, VE, VC and VCE groups, respectively. Vitamin E retention in liver was 25.9, 53.8, 88.2, 124.5 mg/kg in MP, VC, VE and VCE groups, respectively. These results indicate that supplementation of vitamin C and E to moist pellet diet significantly improved growth and feed efficiency of olive flounder, and the mixed addition of vitamin C (1,000 mg/kg dry wt.) and vitamin E (220 mg/kg dry wt.) did not lead to the synergy effect.
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