Abstract

본 연구의 목적은 대나무 줄기표면에서 처음 발견 하였으나, 아직까지는 연구가 미흡한 신 균주 Bacillus amyloliquefaciens NBF11-1의 생리활성 효과를 알아보기 위하여 청국장 전통 발효 균주인 Bacillus subtilis NG24를 대조군으로 하여 항산화효과에 대한 비교분석을 실시하였다. 청국장 추출물의 항산화 실험 결과에서 Total Polyphenol 추출물 함량의 측정 결과는 B.subtilis NG24에 비해 B.amyloliquefaciens NBF11-1 시료에서 유의하게 증가하였다(p=0.032). SOD 유사활성과 DPPH radical 소거능 및 NO radical 소거능에서 B.subtilis NG24에 비해 B.amyloliquefaciens NBF11-1을 포함한 시료에서 농도가 증가함에 따라 유의하게 증가하였다(p<.05). 추가적으로, 각 항산화 실험의 <TEX>$IC_{</TEX><TEX>50}$</TEX>은 B.subtilis NG24에 비해 B.amyloliquefaciens NBF11-1 시료에서 SOD 유사활성(p=0.045), DPPH radical 소거능(p=0.041), NO radical 소거능(p=0.019)과 같이 유의한 차이로 감소하였다. This study aims to compare and analyze the antioxidant effect of Cheonggukjang's traditional fermentation strain, Bacillus subtilis NG24 which was a control of the study, in order to see the biological activity effect of the new strain, Bacillus amyloliquefaciens NBF11-1 that was first found in the surface of the bamboo stem, but hasn't been insufficiently researched. In the antioxidant activity experiment of the Cheonggukjang extract, the B.amyloliquefaciens NBF11-1 sample showed a significant increase in the total polyphenol extract content, compared to B.subtilis NG24(p=0.032). Also, compared to B.subtilis NG24, the sample containing B.amyloliquefaciens NBF11-1 showed a significant increase in SOD-like Activity, DPPH radical scavenging, and NO radical scavenging, as the concentration rose(p<.05). Additionally, <TEX>$IC_{50}$</TEX> in each antioxidant activity experiment significantly decreased in the B.amyloliquefaciens NBF11-1 sample like SOD-like Activity(p=0.045), DPPH radical scavenging(p=0.041), and NO radical scavenging(p=0.019), compared to B.subtilis NG24.

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