Антимікробна активність нового хіміотерапевтичного препарату на основі флюмеквіну щодо Aeromonas Hydrophila

Abstract

In the article presented results of study of the antimicrobial activity of the chemotherapeutic drug on the basis of the active substance – flumequine.The sensitivity of the microflora of the biomaterial to antibiotics was determined and the minimum inhibitory concentrations of flumequin, the active substance of the drug «Flyumek», were determined.For the study were selected 5 fish – carps affected by aeromonosis. The diagnosis for aeromonosis was based on epizootological data, clinical signs of the disease, pathological and anatomical changes and the results of bacteriological research. The fish were slaughtered and crops were made from the affected parts of the skin, liver and kidneys. Sowing was carried out on meat-peptone broth (MPB) and meat-peptone agar (MPA). The seeds were incubated in a thermostat at a temperature of 26 °C for 48 hours. The sensitivity of the microflora of the biomaterial to antibiotics was determined by agar diffusion using standard antibiotic disks and the Müller-Hinton medium. In order to isolate and identify the Aeromonas hydrophila bacterium, the primary culture of the pathological material was performed on the MPA, which was incubated in the thermostat at 26 °C for 24 hours. The next step was to isolate pure cultures of microorganisms and make smears that were stained with Gram in the Björk modification. The method of serial dilutions in a liquid nutrient medium was determined by the minimum inhibitory concentrations (MICs) of flumequine in the preparation of «Flyumek» for Aeromonas hydrophila isolates.The results of the test for the sensitivity of the microflora of the biomaterial from the patient to the aeromonosis of the fish showed high sensitivity of the microorganisms to flumequin, tetracycline, fluorophenicol and trimethoprim, moderate neomycin sensitivity and resistance to amoxicilline, tylosine, lincomycine and colistine. The diameter of the growth retardation zone around the disks with flumequin was – 23,2 mm in the crop sown from the skin, from the kidneys – 30,0 mm, from the liver – 30,4 mm.According to the obtained values of the minimum inhibitory concentrations of flumequine, all investigated isolates of Aeromonas hydrophila were sensitive to the drug «Flyumek» and amounted to 1.0–2.0 μg ml.The results of the conducted studies indicate that fluoroquinolone antibiotic flumequin has a high antimicrobial activity against the bacterium Aeromonas hydrophila, an aeromonosis agent of carp fish.