Взаимосвязь однонуклеотидных полиморфизмов локуса 9р21. 3 с тяжестью атеросклероза коронарных артерий и отдаленными исходами после чрескожной коронарной реваскуляризации у больных инфарктом миокарда

Abstract

Aim. To investigate association between 9p21.3 locus single nucleotide polymorphisms (SNPs) and coronary atherosclerosis severity and long-term outcomes after percutaneous coronary intervention (PCI) in patients with myocardial infarction (MI). Material and methods. A total of 255 Caucasian patients (211 male, 44 female; aged up to 65 years, on the average 52.56±7.98 years) with MI were recruited into the study from 01.01.2009 to 30.06.2010. All participants were included into the study after written informed consent. Genome DNA was extracted from leukocytes of venous blood by the phenol-chloroform extraction method. Two SNPs rs10757278 and rs1333049 (locus 9p21.3) were tested by real-time polymerase chain reaction (PCR) according to protocol (probes TaqMan, Applied Biosystems, 7900HT). The coronary angiograms were reviewed by independent angiographers who were blinded to the results of the genotyp- ing (Philips Allura Xper FD10). The total number of lesions, Gensini score and SYNTAX score were derived. Follow-up lasted two years. Results. Locus 9р21.3 genotypes CC rs1333049 and GG rs10757278 demonstrated a direct strong association with severity of coronary atheromatous burden (left main coro- nary artery stenosis, total number of lesions, Gensini score). There are not influence of locus 9p21.3 on mortality, recurrent MI, hospitalization due to unstable angina, repeated PCI, stroke during follow-up period (6, 12, 24 months). Frequency of the genotype СС rs1333049 among patients with recurrent MI was 20% (without recurrent MI — 27.4%; р=0.54); with hospitalization due to unstable angina — 27.5% (without hospitalization — 26.4%; р=0.82); with repeated PCI — 24.0% (without repeated PCI — 27.2%; р=0.97); among died patients — 29.8% (among survived ones — 26.4%; р=0.76). Frequencies of the genotype GG rs10757278 were similar: recurrent MI (yes — 18.8%; no — 26.4%; р=0.49); hospitalization due to unstable angina (yes — 28%; no — 25.3%; р=0.42); repeated PCI (yes — 23.7%; no — 26.3%; р=0.98); death (yes — 28.6%; no — 25%; р=0.51). Conclusion. Locus 9р21.3 genotypes CC rs1333049 and GG rs10757278 revealed significant association with severity of coronary atheromatous burden in patients with MI. There was no relationship between these genotypes of locus 9р21.3 SNPs and long-term PCI outcomes.