Abstract
Blood level of chloramphenicol in rabbits was measured after oral administration of chloramphenicol crystals or chloramphenicol ester preparation. Estimation of the blood level was made in a following manner. After administration of chloramphenicol, 0.5cc. of blood is drawn, mixed with 0.1cc. of 3.8% sodium citrate, placed in a glass-stoppered test tube, and shaken with 0.2cc. of 1% saponin solution to effect complete hemolysis. To this mixture, 2cc. of the Clark-Lubs buffer (pH 7) and 3cc. of isoamyl acetate are added and the mixture is shaken for 10 minutes to effect extraction. This is centrifuged, 2cc. of the supernatant is mixed with 1cc. each of 3% isonicotinic acid hydrazide solution and 6% sodium hydroxide solution, and shaken in a thermostat of 30° for 30 minutes. The supernatant is decanted and absorbancy of the colored underlayer is measured (filter, S-40; light path, 5mm.). The blood drawn before administration of chloramphenicol is treated in the same manner and its absorbance is measured as the control. Blood level of chloramphenicol is calculated from the calibration curve.
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