Abstract
Mannan of Candida albicans was isolated by modified Vogel's method and purified by preparation of its copper complex. This material showed [α]D+53°, molecular weight of 4500 (Akiya-Barger method and from reducing end-group assay). Its infrared spectrum showed no β-configuration of mannopyranose.Periodate oxidation products of this mannan were identified with glyoxal (as p-nitrophenylosazone, m.p. 310°, and by infrared spectrum) and glyceraldehyde (as p-nitrophenylhydrazone, paper chromatography and electrophoresis). From these results, it was concluded that the mannan has been 1-2, 1-6, or 1-2-6 α-linkages, but not 1-3 or 1-4 linkages.
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