Abstract
The possibility of using trichloroacetic acid instead of the classical mixture of acetic anhydride with sulfuric acid for acetolysis of pollen grains in the melissopalynological analysis of honey is demonstrated. This substance in comparison with other derivatives of acetic acid allows holding the sample in a boiling water bath that improves the result of acetolysis of pollen grains. The possibility of using various solvents for transferring acetolyzed pollen grains to glass slides for further microscopy was also investigated. The optimal reagents and parameters for this analysis were studied and identified during the experiments.
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