Abstract
To prepare an field soil modeled for ecological study of Pythium spp., the field soil was heat-treated with water vapor-saturated air for eliminating native populations of Pythium spp. without disturbing physical and chemical properties of the soil. Oospores of P. spinosum or P. aphanidermatum were then introduced to the treated soil and the untreated soil to observe their germination patterns. These patterns varied with species and seasons, but there was few difference between the soils.
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