Исследование безопасности применения бензилпенициллина натриевой соли после воздействия на нее импульсным магнитным полем

Abstract

Pulsed magnetic fields are widely used in various fields of modern technology. In our previous works it was shown the strengthening of antibacterial properties of benzylpenicillin sodium salt after exposure to a pulsed magnetic field of high tension. The reason for this may be a change in the conformation of β-lactam antibiotics under external physical influence, which increases the affinity of benzylpenicillin molecules to the active center of penicillin-binding proteins. This article presents studies on the safety of benzylpenicillin sodium salt irradiated by a pulsed magnetic field. Benzylpenicillin sodium salt in powdered form was irradiated with a pulsed magnetic field with the intensity H = (0.09∙106-1.23∙106) A/m, frequency f = 30-70 kHz, and a different number of pulses (n = 1-3), using inductors – single-turn and multi-turn. The influence of pulsed magnetic field on chemiluminescence parameters of irradiated benzylpenicillin sodium salt was studied. The appearance of free radicals in irradiated preparations was evaluated in simple model systems simulating the most common free radical oxidation reactions in the body and in environments in which the formation of reactive oxygen species and lipid peroxidation reactions was initiated. Acute toxicity in intraperitoneal administration of antibiotic to mice before and after treatment with pulsed electromagnetic field with calculation and comparison of LD50 indices was also investigated. It was found that the treatment of the dry substance benzylpenicillin sodium salt by a pulsed magnetic field does not cause the formation of free radicals and is not accompanied by an increase in the toxicity of this antibiotic. The possibility of using new ways to increase the antibacterial activity of antibiotics without increasing their concentrations is considered.