ИСПОЛЬЗОВАНИЕ ПЛАЗМЫ, ОБОГАЩЕННОЙ ТРОМБОЦИТАМИ, ПРИ ЛЕЧЕНИИ ОЖОГОВ В ЭКСПЕРИМЕНТЕ

Abstract

Objective. To evaluate the effectiveness of platelet-rich plasma injections in the treatment of wounds in experimental animals with burns. Materials and methods. The experiment was carried out on 30 sexually mature male Wistar rats, which, after simulation of infected burn wound with boiling water and excision of necrotic tissue to pinpoint bleeding, hemostasis, on the second day, were divided into the main and control groups, depending on the chosen strategy of subsequent treatment. Wound defect of the animals in the control group (15 rats) was treated and bandages soaked in 0.02% decamethoxin solution were applied. Platelet-rich plasma was additionally injected into the wound area of the animals in the main group (15 rats) on the 1<sup>st</sup>, 3<sup>rd</sup>, 5<sup>th</sup> day after necrectomy. The remaining plasma was applied to the wound surface followed by closure with a polyvinyl chloride film and gauze bandage. Wound dressings were performed daily. The study involved a histological examination of the wound site and surrounding tissues the collection of which was carried out on the 1<sup>st</sup>, 3<sup>rd</sup>, 7<sup>th</sup>, 14<sup>th</sup> day after the removal of necrotic tissues. Results. In animals of the main group, the treatment of which involved the use of platelet-rich plasma, on the 3rd day after necrectomy in the wound area a decrease in the depth of injury that extended only of to subcutaneous tissue, a decrease in the activity of the inflammatory reaction in the tissues, followed by the proliferation of new epidermis and almost complete healing of wound defect up to 14 days were determined. For comparison, pathological changes in the tissues of the injured area of the animals in the control group were more pronounced with signs of muscle injury. In addition, during the entire observation period in this group of animals, an intense inflammatory reaction with low proliferative activity persisted. Conclusion. The effectiveness of platelet-rich plasma injections as an element of complex local treatment of burn wounds in rats has been experimentally confirmed. What this paper adds The expediency and effectiveness of platelet-rich plasma in the treatment of burns was histologically substantiated in the work on a specific experimental model of rats. In particular, its ability to positively influence the paranecrotic zone, reduce the intensity of inflammatory reaction in tissues, actively restore skin appendages, and stimulate the processes of proliferation and epithelization was established.