Стандартизація сухого екстракту стулок квасолі за вмістом флавоноїдів

Abstract

Introduction. The development of new drugs for treatment and prevention of diabetes is a challenge that is an urgent matter in the context of the increase of diabetes morbidity. The hypoglycemic effect of phaseolus vulgaris pods infusion is established. The development of technology of phaseolus vulgaris pods dry extract, the study of its hypoglycemic effect is possible only by determination of the composition of the extract and its standardization.The aim of the study – to develop methods of phaseolus vulgaris pods dry extract identification; to develop and validate the technique of quantitative determination of flavonoids; to advance the quality criteria of the extract.Research Methods. Qualitative composition of flavonoids was investigated by means of chromatographic methods; quantitative determination was conducted by spectrophotometric technique.Results and Discussion. The research of phenolic compounds of phaseolus vulgaris pods dry extract by chromatographic methods allowed identifying rutin, isoquercitrin, ferulic acid and quercitrin in trace amounts. In addition to the phenolic compounds, the extract contained a large quantity of an unidentified flavonoid, a zone of which on the thin-layer chromatogram was situated between the zones of chlorogenic acid and rutin; two hydroxycinnamic acids, the zones of which were below and above the ferulic acid, and probably were their derivatives. The analysis of electronic spectra of absorption of a standard rutin sample solution and a dry extract test solution within the conditions of quantitative determination with aluminum chloride allowed using rutin as a standard for evaluation of flavonoids content. The content of flavonoids was determined by the spectrophotometric method, it was 0.7-0.8% range expressed in rutin and dry substance for different series of the extract.Сonclusions. To identify the dry extract of phaseolus vulgaris pods it was suggested to choose rutin, isoquercitrin and ferulic acid as active markers. The scheme of the chromatogram, which should be corresponded to the chromatogram of the test solution with the identification of dry extract, was recommended. The content of flavonoids was advised as a quantitative criterion of quality: not less than 0.65% expressed in rutin and dry substance.