ПАРАМЕТРЫ ГУМОРАЛЬНОГО И КЛЕТОЧНОГО ИММУНИТЕТА У САМЦОВ ЯГНЯТ КАРАДИ: ВЛИЯНИЕ ИЗМЕЛЬЧЕННЫХ В ПОРОШОК ЦЕЛЬНЫХ СЕМЯН КАННАБИСА И НАНОСЕЛЕНА

Abstract

Cannabis sativa L. is regarded as a food plant. Whole cannabis seeds typically include 30% oil, 3% saturated fatty acids, and 25% protein. Nano-selenium is a significant element that can perform bioactivity, improve digestibility, and play a significant function in animal immune systems. Owing to the physiological significance of cannabis seeds and nano-selenium, this study examined their effects on cellular and humoral immunological markers. Twenty male Karadi lambs with an average age of 3 to 4 months participated in the study. Four groups of lambs were formed. The second group received 0.5 mg/kg of nano-selenium orally, whereas the first group served as the control group. The third group received 250 mg/kg fed of whole cannabis seed powder, whereas the fourth group received 0.5 mg/kg fed of nano-selenium and 250 mg/kg fed of entire cannabis seed powder orally. To ensure daily ingestion of the precise amount of nano-selenium and cannabis seeds powder, gelatinous capsules were made for the powder of nano-selenium and seeds. The gelatinous capsules were taken orally every day at 9:00 am for 60 days. In the nano-selenium group, eosinophil level was altered significantly on day 45 and lymphocyte on day 60 (p<0.05). Immunoglobulin G in treated groups was significantly altered (p<0.05) compared to the control group on days 45 and 60. Immunoglobulin A in treated groups was significantly decreased (p<0.05, p<0.01) on days 45 and 60 compared to the control group. Immunoglobulin E was significantly increased (p<0.05) only in the cannabis seeds powder group on day 60. No significant change are found in the level of C-reactive protein (CRP) in the nano-selenium group. In contrast, cannabis seeds powder and nano-selenium with cannabis seeds powder were significantly decreased (p<0.01) on days 45 and 60. IL6 and TNF-alpha in cannabis seeds powder significantly declined (p<0.05), while a significant decrement (p<0.05) appeared in IL6 on day 60 in nano-selenium with cannabis seeds powder. In conclusion, using nano-selenium and cannabis seeds and both in combination in mentioned dosage have a positive effect on the immune system, which was the aim of the present study.