Дослідження ембріотоксичної дії препарату для зовнішнього використання «Мазь для ран» на лабораторних тваринах

Abstract

The results of experiments showed one of the aspects of the application safety of the new preparation «Ointment for Wounds», which is intended for external use to bovine cattle, horses, sheep, goats, swine, fur-bearing animals, rabbits, dogs and cats. The preparation formula includes essential oils of Siberian Cedar, Eucalyptus, Tea Tree, Cedar, Clove, and chlorophyllipt oil solution. Pre-clinical trial of the «Ointment for Wounds» were carried out on pregnant rats, through repeated dermal application in the period from the 1st to the 19th day of pregnancy, and it did not cause toxic effects on animals. At the autopsy post-mortem examination of the rats on the 20th day of pregnancy, the quantity of yellow bodies in the ovaries, the implantation and resorption sites in the uterus, the number of live and dead fetuses were estimated. It was defined that the gestation course in control and experimental groups of animals did not differ significantly. The pre-implantation and total embryonic mortality in the experimental group (intervention group) of rats for which «Ointment for Wounds», was used dermally proved to be lower than that of the control group, respectively, by 16.3% and 10.2%. Post-implantation mortality exceeded by 7.0% the similar indicator of the control group. Morphometry of embryos did not reveal the growth retardation of the fetuses. During the external examination, significant visible development anomalies (deformities) of the fetuses extracted from the uterus have not been observed. During the external examination, significant visible development anomalies (deformities) of the fetuses extracted from the uterus have not been observed. Investigation of the fetuses’ internal organs did not reveal defects in their development, as well as defects in the development of the bone system have not been established, that suggests that this preparation does not cause a violation in the osteogenesis processes.The skin application of «Ointment for Wounds» to rats in a dose of 0.5 g/kg in the studied period of pregnancy effects neither on the number of newborns, nor the percentage of stillborns. Parturation of the experimental does took place, as a rule, at the 23rd day of pregnancy, as well as in control group. Consequently, the drug does not cause embryotoxic effects, does not affect the parameters of preimplantation, post-implantation, general embryonic mortality and postnatal development of offspring. Further research will focus on clinical trials of the preparation in the treatment of hyperkeratosis of the dams of the cow dummy.