큰뱀무 추출물의 항산화 활성 및 항노화에 관한 연구

Abstract

In this study, antioxidative effects and inhibitory effects of Geum aleppicum Jacq. extracts on tyrosinase and elastase were investigated. The ethyl acetate fraction of G. aleppicum Jacq. extract (4.70 µg/mL) showed the most prominent free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50). Reactive oxygen species (ROS) scavenging activities (OSC50) of some G. aleppicum Jacq. extracts on ROS generated in Fe 3+ -EDTA/ H2O2 system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction showed the most prominent ROS scavenging activity (0.22 µg/mL). The protective effects of extract/fraction of G. aleppicum Jacq. against the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The G. aleppicum Jacq. extracts suppressed photohemolysis in a concentration dependent manner (1 ~ 25 µg/mL), partic- ularly the ethyl acetate fraction exhibited the most prominent celluar protective effect (τ50, 416.20 min at 10 µg/mL). The inhibitory effect of G. aleppicum Jacq. extracts on tyrosinase and elastase were investigated to assess their whitening and anti-winkle efficacy. The half maximal inhibitory concentration (IC50) of the ethyl acetate fraction on tyrosinase was 95.23 µg/mL. The IC50 of 50 % ethanol extract and the ethyl acetate fraction on elastase were 6.27 µg/mL and 4.31 µg/mL, respectively. These results indicate that extract/fraction of G. aleppicum Jacq. can function as antioxidants in biological systems, particularly skin exposed to UV radiation by scavenging 1 O2 and other ROS, and protect cellular membranes against ROS. Especially the ethyl acetate fraction of G. aleppicum Jacq. ex- tracts could be applicable to new functional cosmetics for antioxidant, antiaging.