Abstract
In this work, the antioxidative effects and active component analysis of Gnaphalium affine D. DON. (G. affine) extracts were investigated. All experiments were performed with 70% ethanol extract, ethyl acetate fraction and aglycone fraction of the G. affine extract. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50) of ethyl acetate fraction (6.15 μg/mL) of the G. affine was higher than that of (+)-α-tocopherol (8.89 μg/mL), which is known as a reference control. Reactive oxygen species (ROS) scavenging activities (OSC50) of the 70% ethanol extract (1.60 μg/mL), ethyl acetate fraction (0.075 μg/mL) and aglycone fraction (2.28 μg/mL) of extract of G. affine on ROS generated in Fe 3+ -EDTA/H2O2 system using the luminol-dependent chemiluminescence assay were much higher than that of L-ascorbic acid (6.88 μg/mL). The cellular protective effects of 70% ethanol extract (τ50 = 52.0 min) and aglycone fraction of the extract (τ50 = 60.6 min) on the 1 O2-induced cellular damage of human erythrocytes were exhibited the higher protective effect than (+)-α-tocopherol (τ50 = 38.0 min), known as a lipophilic antioxidant. TLC and HPLC were used to analyse active components in the aglycone fraction of the extract. Results showed that luteolin 1)
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