Abstract
We report the electro-optical properties of the sensory rhodopsin II using a near-field scanning microwave microscope(NSMM). Rhodopsin was known as a photoreceptor pigment with a retinal as a chromophore via a protonated Schiff base and consists of seven <TEX>${\alpha}-helical$</TEX> transmembrane segments. The sensory rhodopsin II, expressing E. coli UT5600 with endogenous retinal biosynthesis system and purified with <TEX>$Ni^{-2}-NTA$</TEX> affinity chromatography in the presence of 0.02 % DM (Dodecyl Maltoside) from Natronomonas pharaonis. We measured the absorption spectra and the transients difference of sensory rhodopsin II from Natronomonas pharaonis using a UV/VIS spectrophotometer with Nd-Yag Laser (532 nm). The absorption spectra of NpSR II showed a typical rhodopsin spectrum with a left shoulder region and the photointermediates spectra of NpSR II-ground state (<TEX>${\lambda}max=498\;nm$</TEX>), NpSR II-M state (<TEX>${\lambda}max=390\;nm$</TEX>), and NpSR II-O state (<TEX>${\lambda}max=550\;nm$</TEX>) during the photocycle. The observed photocycle reaction was confirmed by measuring the microwave reflection coefficient <TEX>$S_{11}$</TEX> at an operating frequency of f=3.93-3.95 GHz and compared with the results of a photocycle of NpSR II.
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