미나리 추출물의 유리 아미노산 특성과 복어독에 중독된 마우스의 치사 시간에 미치는 영향

Abstract

The purpose of this study is to investigate the characteristics of free amino acids in dropwort (Oenanthe javanica) and get the basic data about detoxication of the natural sources such as dropwort, soybean sprout, ginger, wood sorrel and 4-aminopyridine (4-AP) against the puffer fish poisoning. Dropwort was extracted with hot water and free amino acid in dropwort was analyzed. Asparagine (160.1 mg %), glutamine(119.8 mg %) and γ-aminobutylic acid (36.5 mg %) were major free amino acids (total 417.3 mg %). An aliquot of each extract of dropwort, soybean sprout, ginger, wood sorrel extracts and 4-AP combined with puffer fish toxin was injected to the mice respectively and we have checked the survival time. When only the puffer fish toxin was injected to the mice, the average survival time of mice was 662 second. And as dropwort extract was injected into the mice from 5 to 40 mg, the average survival time of mice was prolonged from 853 second to 1,493 second (Increase life span 232 %, T/C). With the 30 mg of injecting, the results suggested that certain content of free amino acids in dropwort was involved in water soluble antidotal. Another extracts and 4-AP increased survival time considerably depending on the amount injected but not so considerable compared to dropwort extracts.