Abstract
Under the experimental conditions on guinea pigs, we studied the course of reparative skin regeneration after applying a patch wound under the influence of a cell suspension from an autologous stromal-vascular fraction. We used methods of clinical observation, light-optical studies of biopsy material, macro- and microscopic morphometry, and statistical analysis of the data obtained. Cellular material was obtained by liposuction from the withers of the animal. Autologous adipose tissue was used to prepare a cell suspension containing a set of cells (fibroblasts, pericytes, acrophages, fat cells, endothelial cells) that make up the stromal-vascular fraction. On the third day, the stromal-vascular fraction was injected subcutaneously. During the entire experimental period, the animals were under daily observation. Since the introduction of an autologous suspension of cells of the stromal-vascular fraction was carried out on the 3rd day after the application of the wound, a general description for the studied groups was given before this period. On the 7th, 14th and 30th days, planimetric data were obtained, reflecting the change in the dimensional characteristics of the wound surface. The data obtained indicate that the use of an autologous suspension of cells of the stromal-vascular fraction leads to faster (compared to control) healing of a wound skin defect, the formation of a regenerate morphologically similar to the surrounding intact skin. In the structure of the regenerate, the restoration of the structural zones of the skin, such as the epidermis, dermis, and subcutaneous adipose tissue, was microscopically observed. In the central part of the regenerate, a small area of the cicatricial structure remained, the area of which was significantly smaller than that of the control analogs. It is concluded that the use of an autologous suspension of cells of the stromal-vascular fraction enhances the processes of reparative regeneration and is an effective method of treatment in the treatment of skin wounds.
Published Version
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