肝硬変におけるＰｒｏｔｅｉｎ Ｃ活性値による肝機能の評価

Abstract

Immuno-depleted protein C deficient plasma activated by activator venom from Agkistrodon contortrix (Protac®) which is the activator of protein C showed a non-specific amidolytic activity using chromogenic substrate, S-2366. The difference of protein C amidolytic activity between the sample plasma without anti-protein C antibody and with anti-protein C antibody correlated well with the protein C antigen measured by ELISA in 22 normal subjects (r=0.9853, p<0.001). COBAS FARA spectrophotometer programed for a kinetic assay method was used in this study.Using this method the correlations among non-specific cholinesterase (ChE), prothrombin time, thrombotest, antithrombin III activity, protein C amidolytic and anticoagulant activity, protein C antigen and vitamin K-dependent coagulation factors II, VII, IX and X activities were studied in 25 patients with liver cirrhosis. ChE correlated well with prothrombin time (r=0.8178, p<0.001), antithrombin II activity (r=0.7972, p<0.001), factor II (r=0.5550, p<0.01), factor VII (r=0.6299, p<0.001), factor IX (r=0.6379, p<0.001), factor X (r=0.5709, p<0.01), protein C antigen (r=0.7311, p<0.001), protein C amidolytic activity (r=0.7307, p<0.001) and anticoagulant activity (r=0.5504, p<0.02). From these data the level of protein C amidolytic activity and antigen indicates well the liver function of macromolecular synthesis in patients with liver cirrhosis.