Изучение развития арбускулярных микоризных грибов в засоленных почвах Крыма

Abstract

It is known that arbuscular mycorrhiza (AM) fungi are able to mitigate the effect of various negative environmental factors on plants. Therefore, the aim of our research was to determine the most active AM fungi populations in saline soils of the Crimean Peninsula to select stress-resistant isolates. The spores were isolated by wet sieving. Mycorrhization was visualized by staining with black ink and studied quantitatively under a stereomicroscope. In 2019, 15 soil-plant samples from 4 locations of the salinity soils were sampled: Lake Achi (AB) and the nearby agrocenosis (PAB), Koyashskoye (KO), Kirkoyashskoye (PKP) and agrocenosis near it (KP), Chokrak (CH). The highest content of chlorine anions (119.5 mg) and bicarbonate anions (610.0 mg) was noted in the area near Lake Kirkoyashskoye. The smallest salinity was observed at arable land KP (8.5 and 229.0 mg). Plowing led to a decrease in the number of AM fungi spores in the studied sections of PAB by 80.3% and KP by 47.6% compared with the AB and PKP. The frequency of occurrence and the intensity of mycorrhizal colonization in plant roots on the shores of Lakes Achi and Kirkoyashskoye were 70.0-72.3% and 28.0-43.9%, while the same for the plowing areas around them were lower by 28.5-54.1% and 18.0-32.2%, respectively. In our study, there was no strict correlation between the number of spores and the estimated environmental conditions. Soil-plant samples and the spores isolated from them were used as inoculums for obtaining new AM fungal isolates with a stress resistance property.