Abstract
The influence of pulsed magnetic fields (PMF) with different values of field intensity H, discharge current frequency f and a number of pulses n on dermal fibroblasts was studied in vitro. A specialized pulse-magnetic installation «PMI-Bio» and a measuring stand for creating and monitoring the specified parameters of the PMF were created. Four series of experiments with longitudinal and transverse action of the PMF at a field intensity of 3.76⋅106 – 8.7⋅106 A/m (500 and 1000 J) were carried out. It was found that the longitudinal action of the PMF at 500 J does not lead to cell death, preserves all their structural components, and is safe for primary fibroblast cultures. The use of high-intensity pulsed magnetic fields (HI PMF) at 1000 J in both longitudinal and transverse directions leads to a violation of cell adhesion to the culture plastic and changes in the structure of dermal fibroblasts, up to their death.
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