Abstract
The problem of obesity in modern world has a leading place along with other pathologies. Contrary to the settled ideas that the reason of obesity is hypodynamia and overeating, leading medical editions claim that investigating pathology has polymorphic origin and are linked to the cascade of various violations in organs and their systems. Recently collected data array on the basis of which participation of tissue-specific peptide pools in maintenance to a homeostasis is being postulated, in particular, their ability to regulate proliferation processes, differentiation and death of cells is established. We have characterized fractions of low-, medium- and high-molecular-weight protein homogenates from liver, kidney, muscle and adipose tissue samples of rats with experimental obesity. Protein fractions were separated by electrophoresis using the Laemmli method in 10 % PAAG with sodium dodecyl sulfate (SDS). Spreading between proteins in control and group of rats with obesity has showed a difference in quantity of fractions. We have investigated quantitative and qualitative composition of protein fractions in rat's tissues. Under experimental obesity conditions protein composition of tissues changes, the content of median-molecular fraction (67-35 kDa) and low molecular weight fraction (35-10 kDa) increases. Experimental data may indicate connection breach in protein-protein interactions in these viscuses and predict the formation of non-specific proteins and their fragments in the bloodstream, as a result of increased activity of proteolytic enzymes and destruction of viscuses cells. Future studies in specifics of protein composition changes and peptide pool of rat's tissues will improve understanding the biochemical processes under the conditions of this pathology, which is important in the development of new approaches to diagnosis and treatment of obesity principles
Highlights
which entails a number of pathological conditions that create the preconditions for the development of a large number
possible to analyze the nature of the effect
The article considers the possibility of evaluating the preventive effect of natural antioxidants of humic nature
Summary
In response to the action of stress factors of different genesis in a living organism, a cascade of adaptive responses, which can neutralize functional disorders of homeostasis, is triggered. Leukocyte indices in which the parameters of the leukocyte formula were used, allow assessment of the degree of endogenous intoxication, the development of resistance and the character of cellular immunity for the influence of stress factors on the organism of animals and humans.The article considers the possibility of evaluating the preventive effect of natural antioxidants of humic nature on indices of leukocytes on the organism of rats after the influence of combined water-immobilization stress (WICS). The use of natural antioxidant, a feed additive "Humilid" in the pre-season period causes a decrease in the number of leukocytes by 41% compared to values in animals that received purified water during this period and did not differ from those in intact animals. For preventive use of feed additives of humic nature, "Humilid" and "Eco-Impulse Animal" and vitamin E, the studied parameters are close to the values of the control group.
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Schedule a callMore From: Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology
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