植物組織培養によるシンビジウムＰＬＢ増殖へのＣＯ２濃度，光強度および液体培地組成の影響

Abstract

In order to examine the possibility of photoautotrophic growth on the multiplication stage of tissue culture, Cymbidium PLB (protocorm like body) is propagated in various conditions. CO2 (carbon dioxide) concentration, light intensity and liquid medium composition are changed as shown in Table 1. By using shake culture and fermenter system, PLB is grown in MS (Murashige-Skoog) medium.Both shake culture and fermenter system showed similar results. Growth ratio of the dry weight after 14 days of culture in fermenter system to that of the first day is shown in Fig. 2. In cases of no sucrose condition with high CO2 concentration and high light intensity, the growth ratio was more than unity. This clearly indicates the photoautotrophic growth of the PLB because there is no other source of carbon except CO2.As for the amount of the growth, the role of sucrose was remarkable. The PLB grown in the medium with 30g/l sucrose showed 2-5 times higher ratio than the PLB grown under no sucrose condition. In the cases with sucrose, however, higher CO2 concentration and higher light intensity inhibited the growth.Shoots grew actively under low concentration of sucrose even though the growth ratio was low (Fig. 3).