Abstract

본 실험은 생물반응기를 통해 대량증식된 사계성 딸기 조직배양묘의 적정 순화용기를 선발하기 위해 실시하였다. 순화용기는 플라스틱투명용기, 플러그 트레이, I-포트 및 흑색비닐포트 등 4가지를 사용하였다. 토양함수율 10%이상 지속일수는 플라스틱 투명용기가 5일, 흑색비닐포트가 3-4일, 플러그 트레이 2일, I-포트가 1일 순이었다. 생존율은 흑색비닐포트가 80%, 플라스틱투명용기가 70%, I-포트가 55% 및 플러그 트레이가 15% 순으로 나타났다. 또한 생육량은 플라스틱 투명용기가 가장 왕성하였고, 흑색비닐포트와 플러그 트레이는 비슷하였으며, I-포트가 가장 저조하였다. 런너 발생은 흑색비닐포트가 주당 3.3개로 가장 많았으며, 플라스틱투명용기가 2.9개, 플러그 트레이가 1.6개 및 I-포트가 1.2개 순으로 나타났다. 총 생산비용은 흑색비닐포트가 44,405,300원/10a로 I-포트의 51,064,700원/10a보다 6,659,400원/10a이 더 절감되었으며, 총 생산주수도 흑색비닐포트가 102,718주/10a로 I-포트의 72,452.9주/10a보다 30,265.1주/10a 더 생산되었다. 또한 주당 생산비용은 흑색비닐포트가 432.3원으로 I-포트의 704.8원에 비해 272.5원의 감소하였다. 따라서 생물반응기를 통해 대량증식된 사계성 딸기 조직배양묘의 순화용기는 흑색비닐포트가 적당하였다. This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria <TEX>${\times}$</TEX> ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP's (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP's (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

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