Амінокислотний склад сироватки крові та конденсату видихуваного повітря у дітей з бронхіальною астмою

Abstract

Objective: to develop additional non-invasive criteria for diagnosis of bronchial asthma in children from an ecologically unfavourable region on the basis of exploration of the amino acid composition of the serum blood and the exhaled breath condensate. Material and methods. The material for research was the serum of blood and the condensate of expired air, collected in children aged 6-17 years old. In the sampling material the following amino acid composition was determined: lysin, histidin, arginine, asparaginic acid, threonine, serine, glutamic acid, glycine, alanine, cysteine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine and ammonia. In total 48 children from Dneprodzerzhinsk were examined, 28 of them with bronchial asthma and 20 apparently healthy children. The children with asthma were divided into groups depending on the onset of acute asthmatic attack, history of allergies and the severity of the disease. During the examination, all patients were in the stage of clinical remission. Results. The associations of positive family history for allergic reactions in children with bronchial asthma and the serum concentration of glycine and methionine were proved. The detected changes of amino acid composition in the serum and exhaled breath condensate indicate the considerable oxidative stress and specificity of amino acid exchange that is typical for the children with bronchial asthma from an ecologically unfavourable region. Conclusions. Changes in the spectrum of amino acids in the blood serum (glycine and methionine) in children with a positive history of allergies can be an additional prognostic diagnostic criterion for asthma.